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Promega Exonuclease III 
Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.
Supplier: Promega M1815
Includes: Part No. M1811: Exonuclease III 10X Buffer (1 x 1mL); Exonuclease III (5000U).
Part No. M1815: Exonuclease III 10X Buffer (5 x 1mL); Exonuclease III (25,000U).
Description
- A 3′→5′ Exonuclease Specific for Double-Stranded DNA
- Control deletion rate by varying incubation temperature
- Inactivate Exonuclease III by heating at 75°C for 10 minutes
- Supplied at a concentration of 150-200u/μL
Specifications
| 150 to 200 U/μL | |
| 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2 | |
| 7.5 | |
| 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol | |
| Recombinant E. coli strain | |
| Exonuclease III | |
| 50mM NaCl |
| Colorless | |
| >90% | |
| Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA | |
| -30°C to -10°C | |
| 25,000 U | |
| 28000 | |
| Liquid |