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Promega™ Exonuclease III


A 3'→5' exonuclease specific for double-stranded DNA

Manufacturer: promega™  M1815

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Catalog No. PR-M1815

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Description & Specifications

Specifications

Color Colorless
Storage Requirements -20°C
Storage Buffer 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Inhibitors 50mM NaCl
Product Name Exonuclease III
Concentration 150 to 200U/μL
Format Liquid
Source Recombinant E. coli strain
pH 7.5
Molecular Weight 28000
Purity >90%
For Use With (Application) Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Includes 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
Quantity 25,000U