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Promega™ Dual-Glo™ Luciferase Assay System


Generates both firefly and Renilla luciferase luminescence signals

Manufacturer: promega™  E2980

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Catalog No. PR-E2980

 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Description & Specifications

Specifications

Format 96 and 384-well Plates
Quantity 10 x 100mL
Storage Requirements Store Dual-Glo Substrates at -20°C; store Dual-Glo Buffers below 25°C.

Homogeneous reagent system enables fast and simple quantitation of stable luminescence from two reporter genes in a single sample. The activity of the primary reporter is correlated with the effect of specific stimuli and the activity of the co-transfected control reporter provides an internal control to normalize results.

  • Co-reporter minimizes effects of cell number, cell health, transfection efficiency, and nonspecific cellular responses
  • Cells may be assayed for both reporters directly in growth medium—no preconditioning or prelysing necessary
  • Stable signal may be measured for up to two hours after reagent addition—ideal for batch or continuous processing of 96- and 384-well plates
  • Two-step assay is suitable for any luminometer—no on-board injectors required
  • Linear range extends over at least six logs of enzyme concentration for each reporter