Homogeneous reagent system enables fast and simple quantitation of stable luminescence from two reporter genes in a single sample. The activity of the primary reporter is correlated with the effect of specific stimuli and the activity of the co-transfected control reporter provides an internal control to normalize results.
Co-reporter minimizes effects of cell number, cell health, transfection efficiency, and nonspecific cellular responses
Cells may be assayed for both reporters directly in growth medium—no preconditioning or prelysing necessary
Stable signal may be measured for up to two hours after reagent addition—ideal for batch or continuous processing of 96- and 384-well plates
Two-step assay is suitable for any luminometer—no on-board injectors required
Linear range extends over at least six logs of enzyme concentration for each reporter