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Promega™ DPPIV-Glo™ Protease Assay


Homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity

Manufacturer: promega™  G8351

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Catalog No. PR-G8351

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Description & Specifications

Specifications

For Use With (Application) DPPIV Enzyme activity determination, Kinetic studies of DPPIV inhibito r
Includes DPPIV-Glo Buffer (50mL), DPPIV-Glo Substrate (115.2μL), Luciferin Detection reagent (1 Each)
Product Type DDPIV-GLO Protease assay
Quantity 50mL
Storage Requirements -20°C
Sufficient For 500 Assays at 100μL/assay or 1000 assays at 50μL/assay in 96-well plates or 2000 assays at 25μL/assay in 384-well plate

DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. The DPPIV-Glo Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a single DPPIV-Glo Reagent in an “add-mix-measure” format results in DPPIV cleavage of the substrate and generation of a “glow-type” luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of DPPIV activity present. The assay is designed for use with purified enzyme preparations.

  • Homogeneous protocol makes the assay highly amenable to automation
  • More sensitive than fluorescent-based DPPIV assays; the luminescent assay avoids inherent fluorescent background signals and thus provides good signal-to-background readings
  • Assay is linear over more than three logs of DPPIV concentration and can detect less than 1pg/mL enzyme
  • Maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition and is not dependent on accumulation of cleaved product
  • Stable signal allows plates to be read over an extended period of time

DPPIV enzyme activity determination, Kinetic studies of DPPIV inhibitors