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Promega DNA Polymerase I 
DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'→3' direction. Recombinant.
Supplier: Promega M2051
Description
- Catalyzes the Template-Directed Polymerization of Nucleotides into Duplex DNA in a 5′→3′ Direction
- Can be used in a variety of molecular applications
- Is inactivated by heating at 68°C for 10 minutes
- Supplied at a concentration of 5-10u/μL
Specifications
| 5 to 10 U/μL | |
| 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM | |
| 7.5 | |
| 50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol | |
| Recombinant E. coli strain | |
| DNA Polymerase I |
| Colorless | |
| >90% | |
| Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis | |
| -30°C to -10°C | |
| 500 U | |
| Liquid |