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Promega™ DNA-Dependent Protein Kinase


Phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T-antigen and several transcription factors

Manufacturer: promega™  V5811

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Catalog No. PR-V5811

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Description & Specifications

Specifications

Product Name DNA-dependent Protein Kinase
Format Liquid
Color Colorless
Source HeLa nuclear extracts
pH 7.5
Molecular Weight 85 and 70
Includes 400kDa Catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku
Quantity 2500U
For Use With (Application) Phosphorylates several DNA-binding Substrates in-vitro, including the tumor suppressor Protein p53, the SV40 large T-antigen and several transcription factors
Storage Buffer 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% Glycerol
Storage Requirements -70°C

  • Isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa
  • Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C
  • Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol