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Promega™ DeadEnd™ Colorimetric TUNEL System

Provides rapid detection of apoptotic cells in situ at the single cell level

Manufacturer: promega™  G7360

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Catalog No. PR-G7360


Description & Specifications


Quantity 20 Reactions
Format Fluid
Detection Method Colorimetric
For Use With (Application) Detection of apoptotic cells in situ at the single-cell level
pH 6.8
Kit Contents 1 x 20 each Plastic Coverslips, 1 x 4.8mL Equilibration Buffer, 1 x 20mL 20X SSC, 1 x 40μL Streptavidin HRP, 1 x 20μL Biotinylated Nucleotide Mix, 1 x 200μL DAB 20X Chromogen, 1 x 200μ
Storage Requirements -20°C

The DeadEnd Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Biotinylated nucleotides are incorporated at the 3'-OH DNA ends using the enzyme Terminal Deoxynucleotidyl Transferase (TdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown.

  • Modified TUNEL Assay measures nuclear DNA fragmentation
  • Versatile system is compatible with both tissue sections and cultured cells
  • Colorimetric detection is effective in thick tissue sections and enables assessment of cell morphology