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Promega™ CellTiter-Glo™ Luminescent Cell Viability Assay Kit DFS Item

The most sensitive, fastest, homogeneous cell viability assay. Based on luminescent detection of ATP.

Manufacturer:  Promega™ G7572

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Catalog No. PR-G7572

Includes: CellTiter-Glo Buffer; CellTiter-Glo Substrate (lyophilized)



”Add-mix-measure” format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. Data can be recorded using a luminometer or CCD camera 10 minutes after adding reagent.

  • Designed for use with 96- or 384-well plates, making the kit ideal for automated high-throughput screening, cell proliferation assays, and cytotoxicity assays
  • Does not require cell washing, media removal, or multiple pipetting steps
  • Detects as few as 15 cells per well in a 384-well format or 50 cells per well in a 96-well format, reducing the number of cells required per assay
  • Luminescent signal has a half-life generally greater than five hours, depending on cell type and medium used, eliminating the need to use reagent injectors and providing the flexibility for continuous or batch mode processing of multiple plates

Cell proliferation; Cytotoxicity; Cell viability

U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending.
U.S. Pat. No. 7,770,310 and other patents and patents pending.
U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending.
U.S. Pat. No. 7,741,067 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.



For 96 or 384-well plates
1000 × 100μL assays or 4000 × 25μL assays
−30 to −10°C
For automated high throughput screening, cell proliferation assays and cytotoxicity assays, determining the number of viable cells in culture based on the amount of ATP present