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Promega™ CellTiter-Fluor™ Cell Viability Assay

Nonlytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population based on measurement of a conserved and constitutive protease activity within live cells

Manufacturer: promega™  G6080

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Catalog No. PRG6080


Description & Specifications


Quantity 10mL
Storage Requirements -20°C
Detection Method Fluorescence
For Use With (Equipment) 96- or 384-well plates
Kit Contents 1 x 10μL GF-AFC substrate, 1 x 10mL Assay Buffer
For Use With (Application) For normalizing data for live cell number

The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.

  • Can be performed in multiplex with many Promega luminescence assays or spectrally distinct fluorescence assays, eliminating parallel plate processing and reducing cell culture costs
  • Ideal for normalizing data for live cell number, making results more comparable well-to-well, plate-to-plate, and day-to-day