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Promega™ CellTiter 96™ AQueous Nonradioactive Cell Proliferation Assay Kit (MTS)


Provides a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity, or chemosensitivity assays

Manufacturer: promega™  G5430

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Catalog No. PR-G5430

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Description & Specifications

Specifications

Product Type CellTiter 96 AQueous Nonradioactive Cell Proliferation Assay
Detection Method Colorimetric
Storage Requirements -20°C
For Use With (Application) For determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays, cell viability, chemotaxis
Quantity 5000 Assays

Contains a solution composed of a novel tetrazolium compound, MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] and an electron coupling reagent, PMS (phenazine methosulfate). The MTS and PMS solutions are combined and added to cells in culture.

  • Designed to be performed quickly and easily in a 96-well plate with no washing or cell harvesting; detects as few as 1000 cells/well
  • Flexible assay can follow either a five-hour or overnight protocol
  • Eliminates solubilization steps because the colored product is soluble in tissue culture medium
  • Requires no scintillation cocktail, volative organic solvents, or radioactive waste disposal, increasing user safety

Cell proliferation; Cytotoxicity; Cell viability; Chemosensitivity; Cell attachment determinations; Chemotaxis