Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethylketone) is a cell-permeant pan caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and can inhibit induction of apoptosis. For inhibition of apoptosis, Z-VAD-FMK should be added at the same time that apoptosis is induced. Z-VAD-FMK is provided at 20mM in DMSO for convenient addition to cell culture or extracts. The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability. The suggested concentration for use in the anti-Fas mAb-treated Jurkat cell culture model system is 20μM.
Ac-DEVD-CHO is an inhibitor of caspase-3/7 (DEVDase) activity. The concentration of inhibitor required to inhibit caspase activity must be determined empirically for each system. Ten micromolar inhibitor is sufficient to inhibit caspase activity in extracts of apoptotic THP-1 cells.
- Z-VAD-FMK is cell permeable and inhibits apoptosis in situ
- Ac-DEVD-CHO inhibits caspase-3/7 activity