Promega™ Caspase-Glo™ 8 and Caspase-Glo™ 9 Assay Systems
Caspase-Glo™ Assay is a homogeneous luminescent assay that measures caspase-8 or caspase-9 activity.
Manufacturer: Promega™ G8210
The addition of a single Caspase-Glo reagent in an “add-mix-read” format results in cell lysis, followed by caspase cleavage of the substrate, and generation of a stable “glow-type” luminescent signal. The signal generated is proportional to the amount of caspase activity present. The system now includes a separate vial of a protease inhibitor, MG-132 Inhibitor, which may be used to reduce background, thus improving the performance of the Caspase-Glo 9 and Caspase-Glo 8 assays in cell-based applications.
- Utilize a proprietary thermostable luciferase, Ultra-Glo™ Recombinant Luciferase, which improves performance across a wide range of assay conditions
- Do not require sample preparation, sample manipulation, or extended incubation time—maximum sensitivity is achieved in as little as a half hour
- Provide excellent signal-to-noise ratios and superior sensitivity, allowing assays to be performed in 96- or 384-well formats
- Easily automated
Detection of Caspase-8/Caspase-9 activity; Caspase-8/Caspase-9 inhibition determination (IC50); Caspase-8/Caspase-9 activation determination (EC50).
U.S. Patent 6,602,677, Australian Patent 754312 and other patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.
|1 x 1 Bottle Caspase-Glo™ 9 substrate, 1 x 2.5mL Caspase-Glo™ 9 Buffer, 1 x 7.5μL MG-132 inhibitor|
|−30 to −10°C|
|Caspase-9 activity determination, Caspase-9 inhibition determination (IC50), Caspase-9 activation determination (EC50)|
|0.5 to 1 Hour|