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Promega™ Caspase-Glo™ 3/7 Assay Kit


Provides a homogeneous luminescent assay that measures Caspase-3/7 activities

Manufacturer: promega™  G8090

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Catalog No. PR-G8090

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Description & Specifications

Specifications

Quantity 2.5mL
Format Liquid
For Use With (Application) 96- or 384-well plates using either purified Enzyme or cells in culture as sample
pH 7.4
Kit Contents 1 x 2.5mL Caspase-Glo™ 3/7 Buffer, 1 x 1 bottle Caspase-Glo™ 3/7 substrate
Test Time 0.25 to 1 Hour
Storage Requirements -20°C

Utilizes a proluminescent Caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a single reagent optimized for Caspase-3/7 activity, luciferase activity, and cell lysis. Stabilized luciferase improves assay performance across a wide range of assay conditions and makes the assay less likely to be affected by compound interference than other luminescent-, fluorescent-, or colorimetric-based assays.

  • Designed for use with 96- or 384-well plates using either purified enzyme or cells in culture as sample
  • Requires less enzyme or fewer cells than other caspase formats due to low background luminescence
  • Requires no sample preparation, manipulation, or extended incubation times—maximum sensitivity is achieved in as little as one-half to one hour
  • “Add-mix-measure” protocol makes the assay easy to automate
  • Extended-glow signal allows the plates to be read over a three-hour period for batch processing—no injectors required