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Promega™ cAMP-Glo™ Assay


Homogeneous, bioluminescent and high-throughput assay to measure cAMP levels in cells

Manufacturer: promega™  V1502

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Catalog No. PRV1502

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Description & Specifications

Specifications

Quantity 3000 Assays
Storage Requirements -20°C
For Use With (Equipment) For 384-well plates
For Use With (Application) For high throughput analysis of library compounds to identify modulators of GPCR activity
Sufficient For 384 Well plate format

Monitors cAMP production in cells in response to the effects of an agonist or test compound on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

  • Homogeneous assay can be completed in approximately 45 minutes
  • Two steps following lysis of cells
  • Excellent signal-to-noise ratios Signal: Background >200 (with cAMP), >15 (on cells)
  • Easily scalable to 1536-well plate formats and beyond
  • Proven luminescent technology Powered by Ultra-Glo™ Recombinant Luciferase
  • No interference by fluorescent compounds
  • Nonradioactive

High-throughput analysis of library compounds to identify modulators of GPCR activity