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Promega™ cAMP-Glo™ Assay DFS Item

The cAMP-Glo™ Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells

Manufacturer:  Promega™ V1501

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Catalog No. PRV1501



Monitors cAMP production in cells in response to the effects of an agonist or test compound on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

  • Homogeneous assay can be completed in approximately 45 minutes
  • Two steps following lysis of cells
  • Excellent signal-to-noise ratios Signal: Background >200 (with cAMP), >15 (on cells)
  • Easily scalable to 1536-well plate formats and beyond
  • Proven luminescent technology Powered by Ultra-Glo™ Recombinant Luciferase
  • No interference by fluorescent compounds
  • Nonradioactive

High-throughput analysis of library compounds to identify modulators of GPCR activity



300 Assays
For 384-well plates
384 Well plate format
−30 to −10°C
For high throughput analysis of library compounds to identify modulators of GPCR activity