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Promega™ cAMP-Dependent Protein Kinase, Catalytic Subunit


May be used to phosphorylate target proteins or for in vitro enzymological studies of neural and hormonal signal transduction

Manufacturer: promega™  V5161

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Catalog No. PR-V5161

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Description & Specifications

Specifications

Product Name cAMP-dependent Protein Kinase
Format Liquid
Concentration 1.5 to 3mg/mL
Color Colorless
Inhibitors AMP-dependent Protein Kinase Peptide
Source Recombinant E. coli strain
Purity 0.9
pH 6.5
Molecular Weight 40
Quantity 2500U
For Use With (Application) Phosphorylate target Proteins or for in-vitro enzymological studies of neural and hormonal signal transduction
Storage Buffer 350mM Potassium Phosphate (pH 6.8), 0.1mM DTT
Storage Requirements -70°C

  • 40kDa protein purified from a recombinant E. coli strain expressing the catalytic subunit of bovine PKA
  • Purity: 90%
  • Intracellular targets include ion channels, transcriptional activator proteins, and regulatory enzymes of glycogen metabolism
  • Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1pmol of phosphate into casein in one minute at 30°C
  • Storage Buffer: 350mM potassium phosphate (pH 6.8), 0.1mM DTT