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Promega™ BL21(DE3)pLysS Competent Cells


Allow high-efficiency protein expression of genes under the control of a T7 promoter and having a ribosome binding site

Manufacturer: promega™  L1195

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Catalog No. PRL1195

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Description & Specifications

Specifications

For Use With (Application) Allow high-efficiency Protein expression of genes under the control of a T7 promoter and having a ribosome binding site
Includes BL21(DE3)pLysS competent cells >10 x 6cfu/μg
Quantity 20 x 50μL

BL21(DE3)pLysS is lysogenic for λ-DE3 (1), which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase (2) under the control of the lac UV5 promoter. BL21(DE3)pLysS also contains a plasmid, pLysS, which carries the gene encoding T7 lysozyme. T7 lysozyme lowers the background expression level of target genes under the control of the T7 promoter but does not interfere with the level of expression achieved following induction by IPTG.

  • Contains an IPTG-inducible gene for T7 RNA polymerase
  • Time-saving alternative to making competent cells
  • 1mL is sufficient for ten transformations
  • Available in 200μL aliquots
  • Also available for single-use in 50μL aliquots