Promega™ Apo-ONE™ Homogeneous Caspase-3/7 Assay Kit
Highly sensitive, homogeneous fluorescent apoptosis assay for use in cultured cells and with purified caspase. Just add-mix-read.
Manufacturer: Promega™ G7792
Substrate and buffer solutions are combined to make the Apo-ONE Caspase-3/7 Reagent that is added directly to samples. The amount of fluorescent product generated is representative of the amount of active Caspase-3/7 present in the sample.
- May be used with purified enzyme preparations, cell extracts, or cultures of adherent, suspension, or primary cells; compatible with use in cuvets and 96- or 384-well plates
- Simple “add, mix, and measure” protocol and high assay sensitivity decreases time to result; requires fewer cells than comparable caspase assays
- Single reagent addition format is compatible with high-throughput applications
- Apo-ONE Homogeneous Caspase-3/7 Buffer is also available separately
|−30 to −10°C|
|Cuvets, 96- or 384-well plates|
|1 x 1mL 100X Caspase substrate Z-DEVD-R110, 1 x 1mL Apo-ONE™ Homogeneous Caspase-3/7 Buffer|