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Promega™ Apo-ONE™ Homogeneous Caspase-3/7 Assay Kit

Provides a fluorescent method for the fast and sensitive measurement of active Caspase-3 and -7 in a homogeneous format

Manufacturer: promega™  G7790

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Catalog No. PR-G7790


Description & Specifications


Quantity 10mL
Format Cuvets, 96- or 384-well plates
Detection Method Fluorescence
Kit Contents 1 x 100μL 100X Caspase substrate Z-DEVD-R110, 1 x 10mL Apo-ONE™ Homogeneous Caspase-3/7 Buffer
Storage Requirements -20°C

Substrate and buffer solutions are combined to make the Apo-ONE Caspase-3/7 Reagent that is added directly to samples. The amount of fluorescent product generated is representative of the amount of active Caspase-3/7 present in the sample.

  • May be used with purified enzyme preparations, cell extracts, or cultures of adherent, suspension, or primary cells; compatible with use in cuvets and 96- or 384-well plates
  • Simple “add, mix, and measure” protocol and high assay sensitivity decreases time to result; requires fewer cells than comparable caspase assays
  • Single reagent addition format is compatible with high-throughput applications
  • Apo-ONE Homogeneous Caspase-3/7 Buffer is also available separately