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Promega™ AMV Reverse Transcriptase

Catalyzes the polymerization of DNA using template DNA, RNA, or RNA:DNA hybrids

Manufacturer: promega™  M9004

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Catalog No. PR-M9004


Description & Specifications


For Use With (Application) First and second strand synthesis of cDNA, primer extensions and RNA sequencing
Includes 1 x 500μL 5X reaction buffer
Product Name AMV Reverse Transcriptase (HC)
Quantity 600U
Format Liquid
Concentration 20 to 25U/μL
Source Avian Myeloblastosis Virus
Storage Buffer Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50%
Storage Requirements -20°C

  • Ideal for first- and second-strand synthesis of cDNA, primer extensions and RNA sequencing, preparation of labeled hybridization probes, and RT-PCR
  • Well suited for templates with high secondary structures due to its stability at higher reaction temperatures (37° to 58°C)
  • Requires a primer and Mg2+ or Mn2+ for activity
  • Possesses an intrinsic RNase H activity, but is free of contaminating DNases and RNases
  • Stabilized in vitro by nonionic detergents and sulfhydryl compounds

Provided buffer is not compatible with RT-PCR; for RT-PCR, Reverse Transcription 10X Buffer (Mfr. No. A3561) should be used in combination with MgCl2 (Mfr. No. A3511).