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Promega™ AMV Reverse Transcriptase DFS Item

AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

Manufacturer:  Promega™ M5101

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Catalog No. PR-M5101



Description

Description

  • Ideal for first- and second-strand synthesis of cDNA, primer extensions and RNA sequencing, preparation of labeled hybridization probes, and RT-PCR
  • Well suited for templates with high secondary structures due to its stability at higher reaction temperatures (37° to 58°C)
  • Requires a primer and Mg2+ or Mn2+ for activity
  • Possesses an intrinsic RNase H activity, but is free of contaminating DNases and RNases
  • Stabilized in vitro by nonionic detergents and sulfhydryl compounds

Provided buffer is not compatible with RT-PCR; for RT-PCR, Reverse Transcription 10X Buffer (Mfr. No. A3561) should be used in combination with MgCl2 (Mfr. No. A3511).

Specifications

Specifications

10U/μL
Liquid
Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50%
1 x 500μL 5X reaction buffer
300U
First and second strand synthesis of cDNA, primer extensions and RNA sequencing
Avian Myeloblastosis Virus
−30 to −10°C
AMV Reverse Transcriptase
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