ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
High Signal Strength at Low ATP Conversion:Users can measure kinase activity that more closely mimics physiological conditions, making the assay very well suited for low-activity kinases such as receptor tyrosine kinases
Sensitive: The assay is sensitive to low concentrations of ADP, thus requiring less enzyme than other assays and providing cost savings
Universal: The assay can be used with virtually any kinase–enables researchers to screen a wider range of kinases in-house, reducing dependency on costly outsourcing of kinase selectivity profiling
Accurate: Accurately measures ADP levels at a wide range of starting ATP concentrations; users assured that activity measured truly reflects kinase activity and produces accurate IC50s comparable to radioactivity-based assays
Accommodate Wide Range of ATP Levels: The assay can be used at ATP concentrations up to 1mM, important for kinases with high Km values for ATP
Stable Luminescent Signal: Provides users with the flexibility to perform batch plate processing without need for strictly timed incubations
Screen library compounds for effects on target kinases; Profile hits and leads for their potency against target kinases; Substitute for radiolabeled-based assays to check the selectivity of lead compounds against a panel of kinases; Measure activity of immunoprecipitated kinases