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PRKD1 (PKC mu) Recombinant Human Protein DFS Item

PRKD1 (PKC mu) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3791

Catalog No. PV3791



Description

Description

PRKC1 (PKC mu) is a serine⁄threonine protein kinase that is phospholipid-dependant and activated by diacylglycerol and phorbol ester TPA. PRKC1 behaves similarly to most PKC isoenzymes of the PKC family, however, PRKC1 differs in some structural and enzymatic features from the other PKC isotypes, and has been shown to be involved in the signal transduction of a wide range of biological responses.
Specifications

Specifications

NP_002733
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
129.3kDa
75% by SDS-PAGE
–80°C
1 Each
PRKD1 (PKC mu), PKD
PRKD1 was pre-diluted in enzyme dilution buffer and assayed in 12.5mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 67μg/mL MAPKAPK2 peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKD
296nmoles of phosphate transferred to MAPKAPK2 peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 1.67μg/mL
GST Tag
0.38mg/mL
114294, 5587
PRKD1 (PKC mu) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKD1 (PKC mu) Recombinant Human Protein
Kinase assay
Baculovirus
PRKD1 (PKC mu) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.