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PRKCZ (PKC zeta) Recombinant Human Protein DFS Item

PRKCZ (PKC zeta) Recombinant Human Protein

Manufacturer:  Life Technologies LSP2268

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Catalog No. P2268



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCZ (PKC zeta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_002735
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
67.7kDa
75% by SDS-PAGE
–80°C
1 Each
PRKCZ (PKC zeta)
PRKCZ was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL Phosphatidyl Serine, 20μg/mL Diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 200μg/mL Histone H1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinases and inhibitors
PKC Kinase Family
1,100nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 1μg/mL
None
0.53mg/mL
5584, 5590
PRKCZ (PKC zeta) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCZ (PKC zeta) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCZ (PKC zeta) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.