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PRKCQ (PKC theta) Recombinant Human Protein DFS Item

PRKCQ (PKC theta)

$435.00 - $8,330.00

Specifications

Accession Number NP_006248
Assays PRKCQ (PKC theta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Common Name PRKCQ (PKC theta) Recombinant Human Protein
Concentration 0.34mg/mL
Expression System Baculovirus
View More Specs

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Description

Description

The PKC family of serine⁄threonine kinases, including PRKCQ (PKC theta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_006248
PRKCQ (PKC theta) Recombinant Human Protein
Baculovirus
PKC
PRKCQ (PKC theta) Recombinant Human Protein
PRKCQ (PKC theta) Recombinant Human Protein
For research use only. Not for use in diagnostic procedures
Kinase assay
His Tag
PRKCQ (PKC theta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
0.34mg/mL
PRKCQ (PKC theta)
84.4kDa
1 Each
90% by SDS-PAGE
–80°C
Human

For Research Use Only. Not for use in diagnostic procedures.