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PRKCN (PKD3) Recombinant Human Protein DFS Item

PRKCN (PKD3) Recombinant Human Protein

$576.00 - $8,390.00

Specifications

Accession Number NP_005804
Assays PRKCN was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 33μM MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
Common Name PRKCN (PKD3) Recombinant Human Protein
Concentration 0.39mg/mL
Expression System Baculovirus
View More Specs

Products 2
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Description

Description

The PKC family of serine⁄threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_005804
PRKCN (PKD3) Recombinant Human Protein
Baculovirus
PKN
PRKCN (PKD3) Recombinant Human Protein
PRKCN (PKD3) Recombinant Human Protein
For research use only. Not for use in diagnostic procedures
–80°C
Human
PRKCN was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 33μM MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
0.39mg/mL
PRKCN (PKD3), PKC nu
127.9kDa
1 Each
95% by SDS-PAGE
240nmoles of phosphate transferred to MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.83μg/mL
Kinase assay
GST Tag

For Research Use Only. Not for use in diagnostic procedures.