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PRKCN (PKD3) Recombinant Human Protein DFS Item

PRKCN (PKD3) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4044

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Catalog No. PV4044



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCN (PKD3), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_005804
Protein assays and analysis
Frozen
PRKCN (PKD3) Recombinant Human Protein
1mg
For research use only. Not for use in diagnostic procedures
PRKCN (PKD3), PKC nu
PRKCN was pre-diluted in enzyme dilution buffer and assayed in 50mM Tris (pH 7.5), 10mM MgCl2, 0.25mM EGTA, 0.1mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 33μM MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
PRKCN (PKD3) Recombinant Human Protein
Human
0.39mg/mL
Frozen
127.9kDa
95% by SDS-PAGE
–80°C
1 Each
PRKCN (PKD3) Recombinant Human Protein
Kinase assay
PKN
240nmoles of phosphate transferred to MAPKAPK2 kinase peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.83μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.