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PRKCH (PKC eta) Recombinant Human Protein 
PRKCH (PKC eta)
Supplier: Thermo Fisher Scientific P2634
Description
The PKC family of serine⁄threonine kinases, including PRKCH (PKC eta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.Specifications
| NP_006246 | |
| Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 82.2kDa | |
| 20μg | |
| For research use only. Not for use in diagnostic procedures | |
| PRKCH (PKC eta) | |
| PRKCH (PKC eta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
| PRKCH (PKC eta) Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
| 0.33mg/mL | |
| 23683, 5583 | |
| PRKCH (PKC eta) Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| PRKCH (PKC eta) Recombinant Human Protein | |
| PKC | |
| 610nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2μg/mL | |
| His Tag | |
| Kinase and inhibitors | |
| 92% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.