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PRKCH (PKC eta) Recombinant Human Protein DFS Item

PRKCH (PKC eta)

Manufacturer:  Life Technologies LSP2634

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Catalog No. P2634



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCH (PKC eta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_006246
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
82.2kDa
92% by SDS-PAGE
–80°C
1 Each
PRKCH (PKC eta)
PRKCH (PKC eta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
610nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2μg/mL
His Tag
0.33mg/mL
23683, 5583
PRKCH (PKC eta) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCH (PKC eta) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCH (PKC eta) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.