missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

PRKCE (PKC epsilon) Recombinant Human Protein DFS Item

PRKCE (PKC epsilon) Recombinant Human Protein

Manufacturer:  Life Technologies LSP2292

 View more versions of this product

Catalog No. P2292



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_005391.1
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
83.7kDa
90% by SDS-PAGE
–80°C
1 Each
PRKCE (PKC epsilon)
PRKCE (PKC epsilon) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.5), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
5,330nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25 to 2μg/mL
None
0.48mg/mL
5581
PRKCE (PKC epsilon) Recombinant Human Protein
5μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCE (PKC epsilon) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCE (PKC epsilon) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.