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PRKCD (PKC delta) Recombinant Human Protein DFS Item

PRKCD (PKC delta) Recombinant Human Protein

Manufacturer:  Life Technologies LSP2287

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Catalog No. P2287



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_006245
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
77.5kDa
95% by SDS-PAGE
–80°C
1 Each
PRKCD (PKC delta)
PRKCD (PKC delta) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 100μg/mL PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
1,603nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5μg/mL
None
0.98mg/mL
5580
PRKCD (PKC delta) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCD (PKC delta) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCD (PKC delta) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.