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PRKCB2 (PKC beta II) Recombinant Human Protein DFS Item

PRKCB2 (PKC beta II) Protein

Manufacturer:  Life Technologies LSP2251

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Catalog No. P2251



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCB2 (PKC beta II), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_002729
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
77kDa
85% by SDS-PAGE
–80°C
1 Each
PRKCB2 (PKC beta II)
PRKCB2 (PKC beta II) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 100μM CaCl2, 1X lipid mix (100μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 200μg/mL histone H1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
1500nmoles of phosphate transferred to histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25 to 0.5μg/mL
None
0.43mg/mL
26191, 5579
PRKCB2 (PKC beta II) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCB2 (PKC beta II) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCB2 (PKC beta II) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.