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PRKCB1 (PKC beta I) Recombinant Human Protein DFS Item

PRKCB1 (PKC beta I)

Manufacturer:  Life Technologies LSP2291

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Catalog No. P2291



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCB1 (PKC beta I), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_991100.1
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
76.9kDa
95% by SDS-PAGE
–80°C
1 Each
PRKCB1 (PKC beta I)
PRKCB1 (PKC beta I) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 100μM CaCl2, 1X lipid mix (100μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 200μg/mL histone H1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
1,300nmoles of phosphate transferred to histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.34 to 0.67μg/mL
None
0.75mg/mL
5579
PRKCB1 (PKC beta I) Recombinant Human Protein
5μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCB1 (PKC beta I) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCB1 (PKC beta I) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.