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PRKCA (PKC alpha) Recombinant Human Protein DFS Item

PRKCA (PKC alpha) Recombinant Human Protein

Manufacturer:  Life Technologies LSP2227

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Catalog No. P2227



Description

Description

The PKC family of serine⁄threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_002728
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
76.7kDa
95% by SDS-PAGE
–80°C
1 Each
PRKCA (PKC alpha)
PRKCA (PKC alpha) was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 100μM CaCl2, 1X lipid mix (100μg/mL phosphatidylserine, 20μg/mL diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 200μg/mL histone H1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKC
3,271nmoles of phosphate transferred to histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2μg/mL
None
0.47mg/mL
5578
PRKCA (PKC alpha) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PRKCA (PKC alpha) Recombinant Human Protein
Kinase assay
Baculovirus
PRKCA (PKC alpha) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.