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Porcine TNF alpha ELISA Kits, Thermo Scientific™


Measure levels of porcine tumor necrosis factor alpha (TNFa) in pig serum, plasma and cell culture supernatant samples using 96-well plates.

Manufacturer: pierce biotechnology inc  EP2TNFA

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Catalog No. PIEP2TNFA

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Description & Specifications

Specifications

Assay Range 31.3 to 2000pg/mL
Description Porcine TNF alpha ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Precision Dependent upon sample type and level (see instructions)
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity <5pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROa, GROß, IFNg, IFNa, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1a, MI
Sufficient For 96 microplate wells
Total Assay Time 4 hr.
Target Natural and recombinant porcine TNFa (porcine tumor necrosis factor alpha)

Assay TNFα (porcine tumor necrosis factor alpha) levels in pig serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Porcine TNF alpha ELISA Kits.

These porcine TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant porcine TNFα (porcine tumor necrosis factor alpha)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.3 to 2000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Dependent upon sample type and level (see instructions)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GROα, GROβ, IFNγ, IFNα, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1α, MIP-1β, TGFβ, TNFβ or RANTES; mouse TNFα; rat TNFα; bovine TNFβ. This ELISA uses antibodies raised against human TNFα. This ELISA reacts with porcine and human TNFα at 100%

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.