Invitrogen™ PLK4 Recombinant Human Protein
PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases. Recombinant human PLK4 (1-836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Manufacturer: Invitrogen™ LSPV6396
PLK4 localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells.The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
|50mM Tris-HCl pH 7.5, 150mM NaCl, 0.25mM DTT, 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol|
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|Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.|