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Platinum™ Hot Start PCR Master Mix (2X) DFS Item

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup

$92.92 - $1,411.20

Products
Catalog Number Mfr. No. Dye Type No. of Reactions Tracking Dye Price Quantity    

13-000-012

DFS Item
 
invitrogen™
13000012
None 50 reactions None Each for $92.92

13-000-013

DFS Item
 
invitrogen™
13000013
None 200 reactions None Each for $349.65

13-000-014

DFS Item
 
invitrogen™
13000014
None 1000 reactions None Each for $1,411.20

13-001-012

DFS Item
 
invitrogen™
LS13001012
Direct gel loading 50 reactions Green Each for $92.92

13-001-013

DFS Item
 
invitrogen™
LS13001013
Direct gel loading 200 reactions Green Each for $349.65

13-001-014

DFS Item
 
invitrogen™
LS13001014
Direct gel loading 1000 reactions Green Each for $1,411.20
Description & Specifications

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Invitrogen™ Platinum™ Hot Start PCR Master Mix retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability.
Platinum Hot Start PCR Master Mix is available with and without loading dye. Platinum Green Hot Start PCR Master Mix is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Product features:

  • High specificity and increased yields with antibody-mediated hot-start PCR
  • Convenient room temperature reaction setup
  • Versatile formulation for broad range of amplicons
  • Platimun Green Master Mix - Direct gel loading with green PCR master mix
About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, the Taq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).

Using Platinum Hot Start PCR Master Mix
Convenient Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. Platinum Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing

Using Platinum Green Hot Start PCR Master Mix
Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, including DNA sequencing, ligation, and restriction digestion. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.