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Invitrogen™ Platinum™ Hot Start PCR Master Mix (2X) DFS Item

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup

Manufacturer:  Invitrogen™ LS13001012

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Catalog No. 13-001-012


 

Specifications

Description

Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Invitrogen™ Platinum™ Green Hot Start PCR Master Mix retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability. Platinum Green Hot Start PCR Master Mix is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Product features:
  • High specificity and increased yields with antibody-mediated hot-start PCR
  • Convenient room temperature reaction setup
  • Direct gel loading with green PCR master mix
  • Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The polymerase is activated in a temperature-dependent manner (at 94°C) during the start of PCR. Once dissociated from the antibodies, the Taq DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity).

Using Platinum Green Hot Start PCR Master Mix
Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. All you have to do is add template, specific primers, and water. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, including DNA sequencing, ligation, and restriction digestion. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.
Description & Specifications

Specifications

Direct gel loading
1.25 mL Platinum Green Hot Start PCR 2X Master Mix; 1.25 mL Platinum GC Enhancer; 1.25 mL water, nuclease-free
3'-A
5 kb or less
Store at -20°C in a non-frost-free freezer
1 X
50 reactions
Platinum™ Taq DNA Polymerase
Standard
Green