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PKN1 (PRK1) Recombinant Human Protein DFS Item

PKN1 (PRK1) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3790

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Catalog No. PV3790



Description

Description

PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids.
Specifications

Specifications

NP_998725
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
132.1kDa
75% by SDS-PAGE
–80°C
1 Each
PKN1 (PRK1)
PKN1 (PRK1) was pre-diluted in enzyme dilution buffer and assayed in 12.5mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 533μg/mL MAPKAPK2 peptide substrate (KKLNRTLSVA) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
PKN
11nmoles of phosphate transferred to MAPKAPK2 peptide substrate (KKLNRTLSVA) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 8.33μg/mL
GST Tag
0.35mg/mL
10725, 5585
PKN1 (PRK1) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
PKN1 (PRK1) Recombinant Human Protein
Kinase assay
Baculovirus
PKN1 (PRK1) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.