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PIK3CG (p110 gamma) Recombinant Human Protein DFS Item

PIK3CG (p110 gamma) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4786

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Catalog No. PV4786



Description

Description

PIK3CG (p110 gamma) is the sole class IB phosphatidylinositol 3-kinase family member, which along with the class IA family members, generates the important second messenger PIP2. However, unlike the class IA enzymes which signal downstream of receptor tyrosine kinases, PIK3CG primarily signals downstream of GPCRs.
Specifications

Specifications

NP_002640.2
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
130.5kDa
95% by SDS-PAGE
–80°C
1 Each
PIK3CG (p110 gamma), PI3 kinase gamma
PIK3CG (p110 gamma) was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 10μM ATP and 100μM PIP2:PS lipid kinase substrate (PV5100) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 20nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation
Kinase and inhibitors
PI3
34nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. PIK3CG (p110 gamma) activity determined at a final protein concentration of 313ng/mL
His Tag
0.9mg/mL
5294
PIK3CG (p110 gamma) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Lipid kinase
PIK3CG (p110 gamma) Recombinant Human Protein
Kinase assay
Baculovirus
PIK3CG (p110 gamma) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.