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PIK3C2A (PI3K-C2 alpha) Recombinant Human Protein DFS Item

PIK3C2A (PI3K-C2 alpha) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV5586

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Catalog No. PV5586



Description

Description

The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, the Class II enyzmes have been linked to diverse, recpetor-mediated cellular processes including insulin signalling, neuronal survival, and growth factor signalling.
Specifications

Specifications

NP_002636.1
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
PIK3C2A (PI3K-C2 alpha) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
Lipid kinase
PIK3C2A (PI3K-C2 alpha) Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 142-1412
3.74nmoles of ADP formed per minute per mg of total protein using the Adapta™ assay (PV5099). An ADP/ATP standard curve is used to calculate percent conversion of ATP to ADP. PIK3C2A (PI3K-C2 alpha) activity determined at a final protein concentration of 1250ng/mL
GST Tag
0.16mg/mL
171kDa
60% by SDS-PAGE
–80°C
1 Each
PIK3C2A (PI3K-C2 alpha)
PIK3C2A (PI3K-C2 alpha) was diluted in assay buffer and the activity determined in a 10μL Adapta™ assay (PV5099) with 10μM ATP and 100μM PI lipid kinase substrate (PV5371) at room temperature for 1 hour. The reaction was stopped with a 5μL addition of EDTA and detection reagents. The final concentrations are 10mM EDTA, 2nM Adapta™ Eu-anti-ADP antibody and 4nM Alexa Fluor™ 647 ADP Tracer. The plate was read after a 45 min. room temperature incubation
Kinase and inhibitors
PI3
PIK3C2A (PI3K-C2 alpha) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.