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Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis


Ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Manufacturer: thermo scientific™  88701

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Catalog No. PI88701

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Description & Specifications

Specifications

Formulation At least 99% pure enzyme at 250U/uL in 50% glycerol (v/v), 20mM NaCl, 2mM MgCl2, 20mM Tris, pH 8.0
Description Pierce Universal Nuclease for Cell Lysis
Quantity 25kU
Sufficient For 1L lysate

Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.

Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.

Highlights:

  • Broad spectrum degrades all forms of DNA and RNA

  • Highest-quality enzyme nuclease is at least 99% pure, as tested by SDS-PAGE

  • Robust activity 100-fold greater specific activity than DNase I

  • Versatile can be used with a wide variety of cell lysis reagents

Recommended for:

Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing