Thermo Scientific™ Pierce™ Trifluoracetic Acid (TFA)
Bottles of 99.5%-pure TFA (MW 114.02, CAS 76-05-01) liquid (sold by weight); for HPLC use as an ion-pairing agent in reverse-phase peptide separations.
Manufacturer: Thermo Scientific™ PI28903
Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.
For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.
- Purity: >99.5% TFA and exceptional clarity allows sensitive, nondestructive peptide detection at low UV wavelengths
- High-performance packaging: Packaged under nitrogen in amber glass with protective TFE-lined fluorocarbon caps to ensure TFA integrity
- Choice of formats for convenience: 1mL ampules can prepare 1L of 0.1% v/v TFA solution for the mobile phase in reverse-phase chromatography in moments
X in the ordering table indicates that hazardous shipping charges apply.
|Protein biology, protein mass spectrometry analysis, calibration solutions, standards and solvents for mass spectrometry, protein/peptide sequencing, protein/peptide solubilizing agent, amino acid analysis, making 0.1% solutions of trifluoroacetic acid (w/v vs. v/v)|
|98 to 100%|
|20 to 25°C|
|Trifluoroacetic Acid (TFA), 100g, Sequencing Grade, Liquid, Ion Pairing Reagent, High Purity and Exceptional Clarity, High-Performance Packaging, Sequencing Grade, Economical Convenience, Ideal for use in Protein/Peptide Sequencing, Sequencing Grade, Solid-phase Peptide Synthesis|
|Trifluoroacetic Acid (TFA), Sequencing grade|
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