Thermo Scientific™ Pierce™ TiO2 Phosphopeptide Enrichment and Clean-up Kit
Desalt and purify phosphorylated peptides for mass spectrometry using these titanium dioxide spin tips and graphite spin columns with specially formulated buffers.
Manufacturer: Thermo Scientific™ 88303
Thermo Scientific™ Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit enables fast, selective enrichment of phosphorylated peptides for mass spectrometry using titanium dioxide spin tips, graphite spin columns and optimized buffers.
The complete kit includes 24 titanium dioxide (TiO2) spin tips and graphite spin columns with buffers to facilitate preparation of enriched and desalted phosphopeptides for analysis by mass spectrometry (MS).
- Convenient — spin-column format of titanium dioxide and graphite columns enable parallel processing and clean up of multiple samples in less than 2 hours
- High capacity — each column enriches up to 300μg of phosphopeptides
- Complementary — TiO2 enriches a unique set of phosphopeptides that complements the Pierce Fe-NTA IMAC Phosphopeptide Enrichment Kit
- High selectivity — recover phosphopeptides with >90% selectivity
The Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit protocol includes stringent washing conditions that increase the selectivity for phosphopeptides to >95%. Because some of the salts in these stringent washes may still be present in the eluted samples, the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit includes graphite spin columns to desalt and concentrate enriched phosphopeptides, resulting in more successful phosphopeptide analysis results. This kit is compatible with samples digested in solution or after in-gel digestion with trypsin or other MS grade proteases. Using the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit and the Pierce Fe-NTA IMAC Phosphopeptide Enrichment Kits will enable complementary sets of phosphopeptides to be removed from complex samples.
|Pierce TiO2 Phosphopeptide Enrichment Tips|
|Processing 96 digested protein samples (0.3-1mg each) containing up to 100µg of phosphopeptides each|
|200µL pipette tips containing TiO2 resin|