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Thermo Scientific™ Pierce™ SuperSignal West Pico Fast Western Blot Kit

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Manufacturer:  Thermo Scientific™ 35061*

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Catalog No. PI35061



Thermo Scientific™ Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol that allows picogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.

These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate.The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.


  • Fast – picogram levels of detection in about one hour

  • Convenient – no expensive hardware or vacuum required; no clogging issues

  • Simple – optimized protocol makes Western blot analysis easier than ever

  • Economical – no additional consumables or extra equipment to purchase

  • Stable – all kit components are stable for one year stored at 4°C

  • Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody

Compatible with:

Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer



SuperSignal West Stable Peroxide Solution, 100mL, SuperSignal West Pico Luminol/Enhancer Solution, 100mL, Fast Western Rabbit Optimized HRP Reagent, Pico, 20mL, Fast Western 10X Wash Buffer, 200mL, Fast Western Antibody Diluent, 400mL
20 mini blots probed with rabbit primary antibodies
Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate, Rabbit
Multi-component kit
800mL kit