Please login to your online account to display your discounted pricing

Thermo Scientific™ Pierce™ Sulfo-SBED Biotin Label Transfer Reagent


Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Manufacturer: thermo scientific™  PI33033

 View more versions of this product

Catalog No. PI33033

  • / Pack
 

Description & Specifications

Specifications

Chemical Reactivity Amine-to-Nonselective
Cleavable Yes
Description Pierce Sulfo-SBED Biotin Label Transfer Reagent
Formulation Sulfo-N-hydroxysuccinimidyl-2-(6-[biotinamido]-2-(p-azido benzamido)-hexanoamido) ethyl-1,3'-dithioproprionate; powder solid
Membrane Permeable No
Molecular Weight 879.98
PEGylated No
Quantity 10mg
Sufficient For 23mL of typical 1X (0.5mM) reaction solution, or approx. 5 uses at 2mg reagent per use
Spacer Arm Length 14.3; 21.2; 24.7Å
Water Soluble Yes

Thermo Scientific™ Pierce Sulfo-SBED Biotin Label Transfer Reagent is a multifunctional reagent for labeling a purified protein and then covalently transferring the attached biotin tag onto specific interactors of that protein.

Ready-to-use kit eliminates the need to prepare the necessary buffers or purchase the critical reagents necessary to perform a label transfer experiment. Key reagents, Sulfo-SBED and DTT, are supplied in unique, no-weigh, single-use packaging.

Highlights:

  • Sulfo-NHS ester reacts with primary amines at pH 7-9
  • Aryl azide conjugates randomly when activated with UV light
  • Cleavable disulfide bond allows biotin label transfer
  • Discover protein interactions without radiolabeling

Includes:

No-Weigh Sulfo-SBED Label Transfer Reagent, 8 × 1mg; BupH Phosphate Buffered Saline, 1 Pk. (makes 500mL); Label Transfer Buffer (20X), 200mL (makes 4L); Streptavidin-HRP Conjugate, 0.1mg; No-Weigh Dithiothreitol (DTT), 8 × 7.7mg; Slide-A-Lyzer MINI Dialysis Units plus Float, 10K MWCO, 10-100μL, 10 units/Pk.