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Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Manufacturer: thermo scientific™  24525

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Catalog No. PI24525

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Description & Specifications


Chemical Reactivity Carboxyl-to-Amine
PEGylated No
Molecular Weight 217.13
Storage Requirements Store as detailed on product information sheet.
Quantity 5g
Formulation N-Hydroxysulfosuccinimide; powder solid
Water Soluble Yes
Membrane Permeable Yes
Spacer Arm Length
Description Pierce Sulfo-NHS
Sufficient For 4605mL of typical 1X (5mM) reaction solution, or approx. 2500 uses at 2mg reagent per use

Thermo Scientific™ Pierce Sulfo-NHS is pure N-hydroxysulfosuccinimide, the essential compound needed to prepare water-soluble, amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.


  • Forms an acyl amino ester with carboxyl groups when used with the EDC
  • Sulfo-NHS-activated molecule reacts efficiently with primary amines, resulting in stable amide bond
  • Allows for two-step EDC reactions between carboxyl- and amine-containing molecules
  • Sulfo-NHS-activated molecule can be stabilized by drying, allowing manufacture of amine-reactive probes and surfaces
  • Reactive groups: Sulfo-NHS
  • Reactive toward: amino groups (upon activation of carboxylates)