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Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Manufacturer: thermo scientific™  24525

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Catalog No. PI24525

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Description & Specifications


Chemical Reactivity Carboxyl-to-Amine
Description Pierce Sulfo-NHS
Formulation N-Hydroxysulfosuccinimide; powder solid
Membrane Permeable Yes
Molecular Weight 217.13
PEGylated No
Quantity 5g
Sufficient For 4605mL of typical 1X (5mM) reaction solution, or approx. 2500 uses at 2mg reagent per use
Spacer Arm Length
Storage Requirements Store as detailed on product information sheet.
Water Soluble Yes

Thermo Scientific™ Pierce Sulfo-NHS is pure N-hydroxysulfosuccinimide, the essential compound needed to prepare water-soluble, amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.


  • Forms an acyl amino ester with carboxyl groups when used with the EDC
  • Sulfo-NHS-activated molecule reacts efficiently with primary amines, resulting in stable amide bond
  • Allows for two-step EDC reactions between carboxyl- and amine-containing molecules
  • Sulfo-NHS-activated molecule can be stabilized by drying, allowing manufacture of amine-reactive probes and surfaces
  • Reactive groups: Sulfo-NHS
  • Reactive toward: amino groups (upon activation of carboxylates)