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Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

$62.21 - $304.50

Specifications

For Use With (Application) ELISA, WB
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Products
Catalog Number Mfr. No. Formulation Product Name Quantity Price Quantity    

PI37538

 
thermo scientific™
37538
Proprietary protein formulation in phosphate-buffered saline, pH 7.5 StartingBlock (PBS) Blocking Buffer 1L Each for $284.55

EN37539

 
thermo scientific™
37539
Proprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween 20 StartingBlock T20 (PBS) Blocking Buffer 1L Each for $304.50

PI37542

 
thermo scientific™
37542
Proprietary protein formulation in phosphate-buffered saline, pH 7.5 StartingBlock (TBS) Blocking Buffer 1L Each for $284.55

EN37543

 
thermo scientific™
37543
Proprietary protein formulation in phosphate-buffered saline, pH 7.5, with 0.05% Tween 20 StartingBlock T20 (TBS) Blocking Buffer 1L Each for $302.40

PI37579

 
thermo scientific™
37579
Proprietary protein formulation in Tris-buffered saline at pH 7.5 StartingBlock (TBS) Blocking Buffer 100mL Each for $62.21

PI37578

 
thermo scientific™
37578
Proprietary protein formulation in phosphate-buffered saline at pH 7.5 StartingBlock (PBS) Blocking Buffer 100mL Each for $62.21
Description & Specifications

Specifications

For Use With (Application) ELISA, WB

Thermo Scientific™ StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein blocker that provides exceptional nonspecific blocking and broad compatibility with membrane (Western blotting) and 96-well plate (ELISA) methods.

Although no blocking buffer is ideal for every experimental situation, you are likely to obtain excellent results from the start with StartingBlock Blocking Buffers. This is because the blocking agent is performs well with an extremely wide range of antibodies and assay systems. Our scientists have yet to encounter an antibody combination or other protein probing system in which StartingBlock Blocking Buffer is not compatible.

Highlights:

  • Compatible with many detection systems – Western blot, ELISA and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)

  • Short blocking times – less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells

  • Strip and reprobe without reblocking – blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Part No. 21059)

  • High signal:noise ratio – biotin- and serum protein-free formulation contributes to acheiving signal-to-noise ratios from 10:1 to 20:1

  • Convenient formats – available ready-to-use 1X formulations in PBS and TBS, with and without Tween™-20 detergent