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Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

Manufacturer:  Thermo Scientific™ PI37538

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Catalog No. PI37538



Thermo Scientific™ StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein blocker that provides exceptional nonspecific blocking and broad compatibility with membrane (Western blotting) and 96-well plate (ELISA) methods.

Although no blocking buffer is ideal for every experimental situation, you are likely to obtain excellent results from the start with StartingBlock Blocking Buffers. This is because the blocking agent is performs well with an extremely wide range of antibodies and assay systems. Our scientists have yet to encounter an antibody combination or other protein probing system in which StartingBlock Blocking Buffer is not compatible.


  • Compatible with many detection systems – Western blot, ELISA and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)

  • Short blocking times – less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells

  • Strip and reprobe without reblocking – blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Part No. 21059)

  • High signal:noise ratio – biotin- and serum protein-free formulation contributes to acheiving signal-to-noise ratios from 10:1 to 20:1

  • Convenient formats – available ready-to-use 1X formulations in PBS and TBS, with and without Tween™-20 detergent



StartingBlock (PBS) Blocking Buffer
Proprietary protein formulation in phosphate-buffered saline, pH 7.5
30 blots or microplates at 30mL each