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Thermo Scientific™ Pierce™ SM(PEG)n Crosslinkers

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Manufacturer:  Thermo Scientific™ 22104

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Catalog No. PI22104



Thermo Scientific™ Pierce SM(PEG)n is a series of amine-to-sulfhydryl crosslinkers that differ in length from 17.6 to 95.2 Ås as a result of polyethylene glycol spacer arms containing n equals 2 to 24 ethylene glycol units.

The SM(PEG)n reagents are six crosslinkers that have identical amine- and sulfhydryl-reactive groups but differ in length as a result of spacer arms composed of different numbers of ethylene glycol units. Their structures (shown below) have the form NHS-(PEG)n-Maleimide, where n represents 2, 4, 6, 8, 12 or 24 polyethylene glycol (PEG) units. Each SM(PEG)n reagent is produced at the specified length without contamination from analogs of other lengths.The availability of this set of reagents makes it possible to optimize crosslinking experiments to obtain the best conjugate efficiency and function for the intended application or analysis method. Considered together with AMAS (Part No. 22295) and the popular SMCC (Part No. 22360), these reagents allow for amine-to-sulfhydryl crosslinking across spaces ranging from 4.4 to 95.2Å. In addition, the hydrophilic PEG content of SM(PEG)n reagents adds to the aqueous solubility of these crosslinkers and their conjugates. PEG is also less immunogenic than other types of crosslinker spacers, making these reagents ideal for immunogen preparation.


  • Homologous series of increasing spacer arm length, ideal for optimizing conjugation of protein interactions
  • Water-soluble polyethylene glycol (PEG) spacer arms help to maintain or increase solubility of crosslinked proteins
  • Reagents readily dissolve in DMSO for subsequent dilution in aqueous reaction buffers
  • Reactive groups: NHS ester and maleimide
  • Reactive toward: amino and sulfhydryl groups


  • Two ethylene oxide subunits
  • Alternate names: Mal-dPEG2-NHS, NHS-PEG2-Maleimide
  • Molecular formula C18H23N3O9
  • Spacer arm length 17.6Å
  • Mass added to target per crosslink 310.12
  • MW 425.39


  • Four ethylene oxide subunits
  • Alternate names Mal-dPEG4-NHS, NHS-PEG4-Maleimide
  • Molecular formula C22H31N3O11
  • Spacer arm length 24.6Å
  • Mass added to target per crosslink 398.17
  • MW 513.50&


  • Six ethylene oxide subunits
  • Alternate names Mal-dPEG6-NHS, NHS-PEG6-Maleimide
  • Molecular formula C26H39N3O13
  • Spacer arm length 32.25Å
  • Mass added to target per crosslink 486.20
  • MW 601.60


  • Eight ethylene oxide subunits
  • Alternate names Mal-dPEG8-NHS, NHS-PEG8-Maleimide
  • Molecular formula C30H47N3O15
  • Spacer arm length 39.2Å
  • Mass added to target per crosslink 574.27
  • MW 689.71


  • 12 ethylene oxide subunits Mal-dPEG12-NHS, NHS-PEG12-Maleimide
  • Molecular Formula C38H63N3O19
  • Spacer arm length 53.4Å
  • Mass added to target per crosslink 750.38
  • MW 865.92


Pierce SM(PEG)4
195mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
Desiccate in original bottle at -20°C equilibrate the vial to room temperature before opening.
4-unit ethyleneglycol functionalized with succinimidyl and maleimido ends; amber oil or solid